Re: simulator software 
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From: Adam Treister <a...@treestar.com>
Date: Sun Mar 16 2008 - 00:04:42 EDT
Justin, 
We're working hard to finalize
FlowJo Collectors' Edition,
which adds Acquisition
(only for Cytek upgrades). 
That version already has some ability to simulate
acquisition by reading an FCS file as the input,
but 
it ignores the machine settings when simulating
so
increasing the gain doesn't show the increase in
the data previews. 
Making it work interactively in simulation mode
has been postponed
while we smooth out
how the program works
when it's actually acquiring,
but
we'll get you a beta version 
to see if it's a good option for you.
Our intention
is to support simulation 
without needing a license,
so
it will be free for training purposes.
Check out the web page: <CyPod>
On a different note,
I want to thank Allan Kachelmeier, et al
for
putting on another great meeting in Portland this week.
I took several new employees up to
Portland
to expose them what we do,
and
they were all blown away by what cool people
are
doing this interesting science.
Flowers rock.
Adam
Adam Treister
President
Tree Star, Inc.
ISAC CONGRESS Purdue Cytometry Mail List
Various problems regarding advertising and list abuse
Various problems regarding advertising and list abuse
From: J. Paul Robinson j...@flowcyt.cyto.purdue.edu
Date: Mon Mar 31 2008 - 01:18:05 EDT
From J. Paul Robinson - Moderator
Robert is right - there is too much politics and not enough science...
What is happening here, is that there are too many cooks.
Let me make it very clear that we work hard to keep this list clean.
It does not always work. When we identify a failure, we usually
respond to the person concerned and don't waste all your time.
We frequently note in our posts, that advertising is not allowed. 
This list was developed from 2 or 3 individuals who actually had email in 1990, to 3000 over the past 19 years. 
It did not happen by chance, nor was it overnight.
It was developed with a lot of cost ($$$),
a lot of time, 
and
what was a pretty darned good idea when it started. 
We don't tolerate people who try to damage the list.
Now that it's highly successful,
there are a number of individuals that are
trying to either circumvent the list, use it
for their own purposes, or simply sideline it. 
There are even some proposing that they should be able to manipulate the list and its contents in any forum for any purpose.
I am really shocked at this rather callous approach to a scientific discussion board.
I am not making public those individuals or their companies, 
but if I am pushed,
I will identify them publicly.
If I do so, and they create havoc,
it could shut the list down - or end up in a nasty legal battle.
I don't suppose that would be popular?
So,
it seems to me, that we need to get back to basics and focus on the reason this list has been so successful
(and why you all want to use it for advertising - or even why those who what to hijack it...) 
- it does a good if not great job overall.
thanks for your support - all 3000 of you ...well most of you!
Clearly we will provide a mechanism for companies to provide a means for communication....it's on our list...
Maybe I am getting too old for all this abuse....!
regards
Paul Robinson Purdue University
Robert J. Palmer Jr. wrote:
"Ultimately it is Paul's decision since he
and
his crew do all the work to keep this list
what it is,
but
you need to realize that it is
what it is because it is monitored.........."
Yep - so let the moderator do his work.
I believe the moderator indicated that,
when deemed appropriate, certain
"posters" have been unsubscribed;
the only evidence for this was the lack of
subsequent posts.
A grand announcement of rule violation
followed by a public frog-march off the list
seems unnecessary. 
This thread is getting a bit tedious - 
maybe those who wish to continue debate could use the subject line
"vendor posts"
so
the science can be culled from the politics.
Adrian: The only reason it is, as you say,
"occasional announcements"
is because Paul has discouraged this in the past. 
Once you open the door by condoning this
practice why should any of the other
vendors exercise any constraint?
Then you are no longer dealing with an "occasional" announcement.
I am sure all of the companies have
announcements they would like to make to
this group about new locations, websites,
services or products,
but that is what marketing budgets are for.
As
I said previously,
I have no problem with vendors
contributing to any scientific discussions on
the list as I have learned from many
(especially Rick Haugland),
but
announcements of the sort from
Bay Biosciences are marketing.
If everyone thinks this type of post is appropriate then let's be fair
and
let all the vendors have at it and then you'll see how "occasional" it is.
Ultimately it is Paul's decision since he and his crew do all the work to keep this list what it is,
but
you need to realize that it is what it is because it is monitored and commercialism has been discouraged in the past. "JL"
J. Paul Robinson SVM Professor of Cytomics
Professor of Immunopharmacology &
Biomedical Engineering Director, Purdue
University Cytometry Laboratories President, International Society for Analytical Cytology
Purdue University Cytometry Laboratories
Bindley Bioscience Center 1203 West State
Street Discovery Park, Purdue University
West Lafayette, IN 47907-2057 Ph (765) 494 0757; Fax (765) 494 0517 email: j...@flowcyt.cyto.purdue.edu
www.cyto.purdue.edu
Join ISAC - www.isac-net.org
Change lives today -
www.cytometryforlife.org
Received on Mon Mar 31 15:38:00 2008
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Re: clinical flow cytometry
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From: Ryan Farmer <far...@treestar.com>
Date: Mon Apr 14 2008 - 19:05:51 EDT
There is another site of interest along these lines.
Mycyte.org is well established
and
has a variety of online texts, articles
and
other links (protocols, standards, etc.).
Users can expand the variety
and 
amount of information as well. 
It is located at 
http://www.mycyte.org. 
Ryan F 
On Apr 11, 2008, at 10:47 AM, <tolda...@cox.net> <tolda...@cox.net>
wrote:
> There is a brand new on line site available for clinical flow
> resources (books,
> standards, publications and web sites), case studies and more. It is
> in the "wiki" format
> so additional information, articles and cases can be added to expand
> this resource. It
> has some great information.
> It is located at http//wiki.clinicalflow.com
> Teri Oldaker
> ---- Ricardo Morilla <Ricardo.Mori...@icr.ac.uk> wrote:
>> Dear Saleh,
>> There are lots of flow books around, I am not sure if there are any
>> specifically recommended for the exam.
>> For Leukaemia/Lymphoma panels I would recommend the "Atlas of
>> Investigation and Diagnosis"
>> "Lymphoid malignancies" authors Matutes, Bain and Wotherspoon.
>> Distributed in the USA by Clinical Publishing.
>> This covers all laboratory aspects of Lymphoid leukaemia diagnosis
>> including Flow plots an panels as well as pictures of morphology,
>> histopathology cytogenetics and fish.
>> Best wishes.
>> Ricardo.
>> Mr. Ricardo Morilla
>> Institute of Cancer Research.
>> 15 Cotswold Road,
>> Haemato - Oncology Section
>> Brookes Lawley Building
>> Sutton, Surrey.
>> SM2 5NG
>> Te: +44 (0) 20 8722 4259
>> +44 (0) 20 8722 4221
>>>>> "Saleh, Jacqueline" <jackie.sa...@genzyme.com> 09/04/2008 16:58
>> Dear Flowers,
>> We have people in our lab who are interested in taking the flow
>> cytometry certification exam through ASCP.
>> Our lab is a clinical testing lab and our assays are specific for our
>> clinical trial needs. We do not do
>> leukemia/lymphoma markers HIV testing or any type of testing done
>> in a
>> hospital flow lab. I imagine the exam would
>> having questions specific to this type of testing.
>> In order to prepare, I was wondering if people had book
>> recommendations.
>> Also information on
>> what a typical leukemia/lymphoma panel might look like, HIV panels,
>> case
>> studies, examples of DNA and DNA
>> ploidy in relation to prognosis. Any information would be greatly
>> appreciated. Thanks in advance.
>> Jackie Saleh
>> Staff Scientist I
>> Clinical Laboratory Science
>> Genzyme Corporation
>> 1 Mountain Road
>> Framingham,Ma 01701
>> jackie.sa...@genzyme.com <mailto:jackie.sa...@genzyme.com>
>> (508) 271-2972
>> The Institute of Cancer Research: Royal Cancer Hospital, a
>> charitable Company Limited
> by
>> Guarantee, Registered in England under Company No. 534147 with its
>> Registered Office at
>> 123 Old Brompton Road, London SW7 3RP.
>> This e-mail message is confidential and for use by the addressee
>> only. If the
> message is
>> received by anyone other than the addressee, please return the
>> message to the sender by
>> replying to it and then delete the message from your computer and
>> network.
Ryan Farmer
TreeStar Inc. 
340 A St. Suite 301
Ashland, OR. 97520
far...@treestar.com 
(541) 201-0022 ext. 172
www.treestar.com
www.flowjo.com 
www.flowdx.com 
www.mycyte.org
Received on Tue Apr 15 13:58:00 2008
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How to Create Corporations and keep Vendors out of the the LUCRATIVE
CLINICAL market.
Purdue Cytometry Mail List Plans to have Isac Congress Persuade the
FDA to FORCE their FCS STANDARDS!
From: Randy T. Fischer (fisch...@vax.grc.nia.nih.gov) Date: Thu Jul 17
1997 - 03:25:15 EST * Next message: kc...@samsung.co.kr: "LDL Receptor
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________________________________________
I agree with both Marty and Gunter in the very important issue of
standardizing data formatting.
I would point out that lobbying ISAC is only, however, part of the
answer. Regardless of what ISAC may choose to recommend, it is still
up to the manufacturers to implement what they want to do,
and if they do not agree with ISAC, then too bad for ISAC
and the flow community.
A potentially more powerful force for change might be the
FDA, which regulates machines used in CLINICAL settings.
If the FDA could be persuaded
to require all CLINICAL data be universally both accessible and
readable, then the manufacurers would be forced to upgrade machines
and software
or
lose theLUCRATIVE CLINICAL market.
This would make anlyzing data from different sources easier,
and could facilitate the exchange of crucial clinical results from
various trials where multiple sites and machines are in use.
So how does this get done?
Gunter (and Paul's agreeing response) are correct this needs to be
revisited at Asilomar, with perhaps an additional idea.
Any concrete standardization protocol, FCS3.0 or whatever it ends up
being designated, should be then presented to any and all regulatory
agencies by ISAC to ensure no individual manufacturer decides FCS3.0
in their format is acceptable, even if it is not universally
readable.
Randy T. Fischer NIA/NIH GRC Baltimore, MD 21224
fisch...@vax.grc.nia.nih.gov
Now your Leaders of ISAC Congress and the Data Standards Committee
A must read
Collusion?
http://www.facebook.com/note.php?note_id=23879772873&ref=mf