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ISAC Congress & Purdue Cytometry Mail List Overdue for Congressional Investigation

ISAC Homepage - ISAC E-News -- March 2008
Mar 13, 2008 ... Since the Society’s inception, we have developed a
mailing list which .... Mark Munson, Verity Software House, Inc., E-
mail: sa...@vsh.com. ...
www.isac-net.org/content/view/732/44/ - 53k - Cached - Similar pages
-
Note this

The Daily Dongle
Cytometry Developers Workshop is attended by the industry and academia
alike. ... J Paul Robinson delivered an effective summary of the
problem, ...
flowjo.typepad.com/ - 40k - Cached - Similar pages - Note this ...

Joseph Robinson: ZoomInfo Business People Information
... Paul Robinson, chair of the Optics East Life Sciences symposium **********
(see sidebar) and director of Purdue University Cytometry Laboratories
(West Lafayette, ...
www.zoominfo.com/people/Robinson_J._-82453.aspx - 24k -

International Congress Montpellier 2004
File Format: PDF/Adobe Acrobat - View as HTML
Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of
Plants .... analytical cytometry publishing and software companies,
pharmaceuti- ...
www.afc.asso.fr/actu/congres/isac04.pdf - Similar pages - Note this

Africa Business Conference 2007
J. Paul Robinson Director, Purdue University Cytometry Laboratories
and Deputy ... in bioengineering with hardware and software groups
developing innovative ...
www.hbsafricaconference.org/2007/panels_healthcare.html - 27k - Cached
- Similar pages - Note this

Review Members and Corporations on the Purdue Cytometry Mial list

Photonics: Principles and Practices - Google Books Resultby Abdul Al-

Azzawi - 2006 - Science - 968 pages
Newport Corporation. Optics and mechanics. Newport 1999/2000
catalog, ... Robinson. Paul, Laboratory Manual to Accompany
Conceptual
Phvsics. ...
books.google.com/books?isbn=0849382904...

Purdue Cytometry Mailing List: RE: EPICS C data
From: J.Paul Robinson
(j...@flowcyt.cyto.purdue.edu) ..

. From: Suzanne Leif > President of ********
Newport Instruments > Via Robert C. Leif, Ph.D. Vice President ...
www.cyto.purdue.edu/hmarchiv/1998/1995.htm - 7k - Cached - Similar
pages - Note this
Dayong JinJingli Yuan’s group),
Newport Instruments **************
(Dr. Robert Leif’s group) and
Purdue University Cytometry Labs
(Prof. J.Paul Robinson’s group). ... ******

www.ics.mq.edu.au/gen/person/jin.html - 13k - Cached - Similar pages
-
Note this
Data and Image Analysis Special Interest Group Meeting 2007J. Paul
Robinson, SVM Professor of Cytomics, Purdue University and
President, ... (*) Robert C. Leif, Newport Instruments. "Cytometry
Standards Continuum" ...
www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages -
Note this

[PPT] DIA SIG 2007: What are the Issues?File Format: Microsoft
Powerpoint - View as HTML
... J. Paul Robinson, President, International Society for Analytical
Cytology, ... (*) Robert C. Leif, Newport Instruments "Cytometry
Standards Continuum" ...
www.ravkin.net/SBS/DIA%20SIG%202007%20Intro.ppt - Similar pages -
Note
this

Wiley Cytometry - ISAC 2000 International Congress POSTER
ABSTRACTS
Stephanie Ann Sincock,
Purdue University; J.Paul Robinson,
Purdue University ...... Robert Leif, Newport Instruments; John
Quagliano, Los Alamos National ...
www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters...
- 223k - Cached - Similar pages - Note this

Verity Software House, Inc.
10 New Lewiston Road
Topsham, Maine 04086
207 729 6767 voice
207 729 5443 fax

Phoenix Flow Systems
11575 Sorrento Valley Road, Suite 208
San Diego, CA 92121
619 453 5095 voice
619 259 5268 fax

Cytomation, Inc.
400 E. Horsetooth Rd., Suite 100
Fort Collins, CO 80525
800 822 9902 voice
303 226 2322 fax NEW ADDRESS FORT COLLINS DRIVE

DOES THE FEDERAL GOVERNMENT REGULATE ISAC CONGRESS ...

A numerical recipe for accurate image reconstruction from discrete ...
- 2 visits - Nov 28
J. Paul Robinson, Bindley Bioscience Center, Purdue University, 1203
West State St., West Lafayette, IN 47907, USA and Cytometry
Laboratories, ...
portal.acm.org/citation.cfm?id=1221224 - Similar pages - Note this

Ø [PDF]
> GREAT LAKES INTERNATIONAL IMAGING AND FLOW CYTOMETRY
> ASSOCIATION
> File Format: PDF/Adobe Acr
> obat - View as HTML
> J. Paul Robinson, Philip Marder. Iowa. Bruce Pesch. Michigan .... flow
> cytometry software. Clearly, traditional cytometers can not handle the
> demands of HTS ...
> gliifca.org/pdf/GLIIFCA-2001.pdf - Similar pages - Note this
> International Congress Montpellier 2004
> File Format: PDF/Adobe Acrobat - View as HTML
> Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of
> Plants .... analytical cytometry publishing and software companies,
> pharmaceuti- ...www.afc.asso.fr/actu/congres/isac04.pdf- Similar pages - Note this

Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED)
... Verity's MODFIT software has a very nice tool for
proliferation ... Stelekati [mailto:e...@fz-borstel.de] >>>Sent:
Monday, March 26, 2007 6:58 AM >>>To: Cytometry Mailing List

>>>Subject: CFSE graphs >>> >>>Dear all, >>>I want to ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0490.htm - 9.0KB
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30 Mar 07

Purdue Cytometry Mailing List: 31st Annual Flow Cytometry Course ...
From : Mark - Verity Software House <m...@vsh.com> ... and lectures
you'll learn about pertinent topics in the art and science of flow
cytometry. ...
www.cyto.purdue.edu/hmarchiv/Current/0455.htm - 8k - Cached - Similar
pages - Note this

Purdue Cytometry Mailing List: ModFit LT 3.1 Service Pack 1 now ...
For more information, contact Verity at sa...@vsh.com. Best regards,
Mark Mark E. Munson Sales Manager Verity Software House, Inc. 45A
Augusta Road PO Box ...
www.cyto.purdue.edu/hmarchiv/2003/1134.htm - 6k - Cached - Similar

From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>

Date: Mon Mar 31 2008 - 01:18:05 EDT

From J. Paul Robinson - Moderator

Robert is right - there is too much politics and not enough
science...

What is happening here, is that there are too many cooks.

Let me make it very clear that we work hard to keep this list clean.
It
does not always work. When we identify a failure, we usually respond
to
the person concerned and don't waste all your time.

We frequently note in our posts, that advertising is not allowed.
This
list was developed from 2 or 3 individuals who actually had email in
1990, to 3000 over the past 19 years. It did not happen by chance,
nor
was it overnight. It was developed with a lot of cost ($$$), a lot of
time, and what was a pretty darned good idea when it started. We
don't
tolerate people who try to damage the list.

Now that it's highly successful, there are a number of individuals
that
are trying to either circumvent the list, use it for their own
purposes,
or simply sideline it. There are even some proposing that they should
be
able to manipulate the list and its contents in any forum for any
purpose. I am really shocked at this rather callous approach to a
scientific discussion board. I am not making public those individuals
or
their companies, but if I am pushed, I will identify them publicly.
If
I
do so, and they create havoc, it could shut the list down - or end up
in
a nasty legal battle. I don't suppose that would be popular?

So, it seems to me, that we need to get back to basics and focus on
the
reason this list has been so successful (and why you all want to use
it
for advertising - or even why those who what to hijack it...) - it
does
a good if not great job overall.

thanks for your support - all 3000 of you ...well most of you!

Clearly we will provide a mechanism for companies to provide a means
for
communication....it's on our list...

Maybe I am getting too old for all this abuse....!

regards

Paul Robinson
Purdue University

Collusion is an agreement, usually secretive, which occurs between
two
or more persons to deceive, mislead, or defraud others of their legal
rights, or to obtain an objective forbidden by law typically
involving
fraud or gaining an unfair advantage. It can involve "wage fixing,
kickbacks, or misrepresenting the independence of the relationship
between the colluding parties."[1] All acts affected by collusion are
considered void.[2]

In the study of economics and market competition, collusion takes
place within an industry when rival companies cooperate for their
mutual benefit. Collusion most often takes place within the market
form of oligopoly, where the decision of a few firms to collude can
significantly impact the market as a whole. Cartels are a special
case
of explicit collusion. Collusion which is not overt, on the other
hand, is known as tacit collusion.

According to neoclassical price-determination theory and game theory,
the independence of suppliers forces prices to their minimum,
increasing efficiency and decreasing the price determining ability of
each individual firm. However, if firms collude to increase prices
loss of sales is minimized as consumers lack alternative choices at
lower prices. This benefits the colluding firms at the cost of
efficiency to society.

One variation of this traditional theory is the theory of kinked
demand. Firms face a kinked demand curve if, when one firm decreases
its price, other firms will follow suit in order to maintain sales,
and when one firm increases its price, its rivals are unlikely to
follow, as they would lose the sales' gains that they would otherwise
get by holding prices at the previous level. Kinked demand
potentially
fosters supra-competitive prices because any one firm would receive a
reduced benefit from cutting price, as opposed to the benefits
accruing under neoclassical theory and certain game theoretic models
such as Bertrand competition.

Practices that facilitate tacit collusion include:

Uniform prices

A penalty for price discounts

Advance notice of price changes **************************

Information exchange ******************

From: Adam Treister (a...@treestar.com)
Date: Mon Apr 22 2002 - 00:15:07 EST
* Reply: Mario Roederer: "Job Opening -- Immediate -- Vaccine

______________________________________...
Only two more weeks until ISAC, that biennial bacchanalia of flower
power
and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.

At the show we'll be releasing FlowJo Version 4. We've got new
platforms
for overlaying and clustering, we've made it work better across the
Internet, added all sorts of new

For those who haven't found it yet, we've unveiled a spanking new
FlowJo
website. Flowjo.com is chuck full of new content, functionality and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new
depths of understanding, and none of that awful yellow on black text.
The
search engine even works. No ads & cookie-free. Check it out.
*************
Specifically, you should check our pricing. Prices are going up on
*******
May 10. ************
It has been a number of years since we've changed our prices and with
the
development of the OSX and PC versions, it¹s time for a leap. I
guess
there's no such thing as a free launch. Anyway, this may be a great
time to
buy that FlowJo ten pack you've been thinking about. Licenses
purchased
before May 10 are entitled to a year of free upgrades, including the
4.0
release.
Unleash the flower power!
Adam
--
Adam Treister
Tree Star,

Recent FlowJo announcement

From: Steve Kelley
(SKEL...@flowcyt.cyto.purdue.edu)
Date: Wed Dec 30 1998 - 06:13:04 EST

Next message: Steve Kelley: "Possible minor disruption"
Previous message: Mark A. Corio: "Chemdex no help..."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
I know that many people on the mailing list are adamantly opposed to
anything that even looks a little commercial, and to try to forestall
any possible

complaints about the FlowJo announcement
, I'll explain what

I'd like to see. In my opinion, announcements about new products, and
product updates are completely appropriate as long as they aren't
abused.
I've never wanted to specifically encourage that, because I really
don't want to be put into a position of having to decide whether a
message is an

"announcement"
or an

"advertisement"
. The companies involved in our cytometry community have always been
extremely good 'citizens' as far as I can tell.

We have representatives of many companies on the list, and they have
always had the power to make my life miserable, and the list no more
than junk mail. Instead, they have helped build this mailing list
into one of the most useful around, through their contributions and
responses. I'm not going to try to set specific rules about what
people can say about their own products, and when they can say it.
I'll just ask that everyone continue to show restraint; that the
commercial representatives ask themselves before they submit an
announcement whether they'd mind seeing every other company in the
business sending the same message they are about to, and that the
non-
commercial (and anti-commercial) people accept discreet announcements
as simple information, and continue the sometimes brisk discussions
about problems and benefits of particular products, alongside the
purely scientific (and occasionally purely entertaining)
conversation.
Steve Steve Kelley

From: Bushnell, Timothy <Timothy_Bushn...@URMC.Rochester.edu>
Date: Fri Mar 21 2008 - 07:21:57 EDT

Colleagues:

As you probably know, ISAC is holding elections this year. The
deadline
for voting is next Wednesday, March 26th. This was announced via
email
and on the ISAC homepage at:
http://www.isac-net.org/content/view/730/2/ .

I am a candidate for Biological Councilor, and would like to share
with
you my thoughts on my goals and plans should I be elected. I would
welcome anyone's comments on these goals because, as Bob pointed out,
with discussion we can refine our positions and be responsive to you,
the members of ISAC.

What I hope to bring to ISAC is an expansion of the existing programs
which serve to unite and coordinate existing affiliated societies in
North America and abroad. ISAC is uniquely positioned to support the
growth and development of regional meetings and other events to
facilitate professional networking and contacts directly related to
cytometry. Networking at such meetings is a very effective way of
communicating and sharing pertinent information about cytometry -
often
to those not affiliated with ISAC - which will increase membership as
the benefits of ISAC association are presented to an untapped market.

These meetings can also serve as a clearinghouse to discuss and
promulgate the ISAC initiatives including: education, data
presentation,
data file standardization, and biosafety. Getting this information in
the hands of the casual user will greatly enhance ISAC's efforts to
affect wholesale change in the scientific community.

I'm very enthusiastic about adding my efforts to the ISAC - most
specifically areas I have expertise in:

* Expanding the membership base in
North
America and abroad by developing ISAC initiated outreach programs, at
the local and regional level. These programs will seek to identify
the
casual user of cytometry, and provide them with the methods,
protocols
and standards that ISAC has and will continue to develop.

* Finding, recognizing and recruiting
cytometry facility core directors as a group vital to our membership
and
supporting them by offering ISAC models, standards, information, as
well
as an international resource to which they can come with problems,
questions and issues.

* Supporting and building more
regional
meetings with a greater ISAC presence via an information kiosk,
financial support for speakers, and a level of professional
camaraderie
that has been the hallmark of cytometry users around the world.

* Building stronger, mutually
beneficial
alliances with corporate sponsors at a regional level to facilitate a
growing base of new and existing regional groups.

My experience in developing and growing the WNYFUG from nothing, as
well
as my training as a member and program chair of the long-running
GLIIFCA
meeting provides me the perspective and skill-set necessary to
achieve
these goals.

I am an avid supporter of technology, specifically cytometry based
instrumentation, for the greater understanding of biology across the
board - however, with such change and development as we've seen in
just
20 years, that needs to be tempered with sound practices, strength of
information, networking on a global scale so that we are keenly aware
of
both developments in cytometry as well as potential pitfalls. The key
to
this level of awareness on a global scale is by seeing and serving
the
regional areas, where those 'in the trenches' can find guidance and
direction from those around the world.

I would urge all ISAC members to take a few minutes to review the
candidates and cast a vote. Your selection helps shape the future of
the Society.

Thank you in advance for your support,

Tim Bushnell

Timothy Bushnell, Ph.D.

Research Assistant Professor, Pediatrics and Oncology

Co-Director, URMC Flow Cytometry Facility

Office: 585-273-5535

Lab: 585-273-1361

Cell: 585-690-5157

Fax: 585-276-0233

http://www.urmc.rochester.edu/Aab/geneped/flow/

http://www.urmc.rochester.edu/wnyfug/

Received on Fri Mar 21 15:58:00 2008

ISAC Congress Executive Joanne Lannigan

joannelanni.._at_virginia.edu with questions

University of Virginia's Site License for FlowJo

http://www.healthsystem.virginia.edu/internet/cyto ...

FlowJo Site License
Registration Form

Complete the form below to access the University of Virginia's Site
License for FlowJo data analysis software. All fields with * must be
completed or your registration will not be processed. Once your
information has been received and verified you will receive a serial
number which you must enter in FlowJo on the computer whose hardware
address you provided. Cost per user license will be determined at the
end of the year depending on the average number of registered users
for the year. (See instructions for pricing structure) This amount
will be billed to the PTAO provided below, so please make sure the
PTAO you provide does not expire before that time. Completion of this
registration form is a valid request for services and assumes the
approval of the Principal Investigator of the PTAO account provided.
Cont
act
Joanne Lannigan joannelanni..._at_virginia.edu with questions.

*****************************************************************************************************

GLIIFCA 2007-2008 Officers and Committees

President and Scientific Program Chair Paul Wallace

Program Committee Members Mike Sramkoski, Vera Donnenberg

Executive Secretary
Co-secretary

Mary Paniagua
Ryan Duggan*
Treasurer Brian K. DuChateau
Education and CMLE Chairs Jonni Moore***********
Vendor Liasons: Karen Domenico, Tom Sawyer
Website Manager Tim Bushnell* *******************

STEERING COMMITTEE MEMBERS
Canada David Hedley , Betty-Anne McBey,Nicole McFarlane
Illinois Charles Goolsby , Maurice R. G. O'Gorman , Mary
Paniagua,
Ryan Duggan*
Indiana Lisa Green ,** Bartek Rajwa********************
Iowa Bruce Pesch , Kristi Harkins
Massachusetts Betsy Ohlsson-Wilhelm, Brian K. DuChateau
Michigan Alexander Nakeff , Louis King
Minnesota Paul Champoux
New York Paul Wallace , Timothy Bushnell************************
Ohio Karen Domenico , Tom Sawyer , Mike Sramkoski
Pennsylvania Jonni Moore *, Vera Donnenberg
Virginia Joanne Lannigan**************************************
Wisconsin Kathy Schell , Kathy A. Muirhead , Matt Hanson
Emeritus Carleton C. Stewart, Sigrid Stewart

Review the Executive Members on ISAC Congresss

Re: clinical flow cytometry

This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ] [ In reply
to ] [ Next in thread ]
From: Ryan Farmer <far...@treestar.com> *********** WEBMASTER

Date: Mon Apr 14 2008 - 19:05:51 EDT

There is another site of interest along these lines.

Mycyte.org

is well established and has a variety of online texts, articles and
other links (protocols, standards, etc.). Users can expand the
variety
and
amount of information as well.

It is located at http://www.mycyte.org.

Ryan F ********WebMaster Mycyte.org

On Apr 11, 2008, at 10:47 AM, <tolda...@cox.net> <tolda...@cox.net>
wrote:

> There is a brand new on line site available for clinical flow
> resources (books,
> standards, publications and web sites), case studies and more. It is
> in the "wiki" format
> so additional information, articles and cases can be added to expand
> this resource. It
> has some great information.
> It is located at http//wiki.clinicalflow.com
> Teri Oldaker
> ---- Ricardo Morilla <Ricardo.Mori...@icr.ac.uk> wrote:
>> Dear Saleh,
>> There are lots of flow books around, I am not sure if there are any
>> specifically recommended for the exam.
>> For Leukaemia/Lymphoma panels I would recommend the "Atlas of
>> Investigation and Diagnosis"
>> "Lymphoid malignancies" authors Matutes, Bain and Wotherspoon.
>> Distributed in the USA by Clinical Publishing.
>> This covers all laboratory aspects of Lymphoid leukaemia diagnosis
>> including Flow plots an panels as well as pictures of morphology,
>> histopathology cytogenetics and fish.
>> Best wishes.
>> Ricardo.
>> Mr. Ricardo Morilla
>> Institute of Cancer Research.
>> 15 Cotswold Road,
>> Haemato - Oncology Section
>> Brookes Lawley Building
>> Sutton, Surrey.
>> SM2 5NG
>> Te: +44 (0) 20 8722 4259
>> +44 (0) 20 8722 4221
>>>>> "Saleh, Jacqueline" <jackie.sa...@genzyme.com> 09/04/2008 16:58
>> Dear Flowers,
>> We have people in our lab who are interested in taking the flow
>> cytometry certification exam through ASCP.
>> Our lab is a clinical testing lab and our assays are specific for our
>> clinical trial needs. We do not do
>> leukemia/lymphoma markers HIV testing or any type of testing done
>> in a
>> hospital flow lab. I imagine the exam would
>> having questions specific to this type of testing.
>> In order to prepare, I was wondering if people had book
>> recommendations.
>> Also information on
>> what a typical leukemia/lymphoma panel might look like, HIV panels,
>> case
>> studies, examples of DNA and DNA
>> ploidy in relation to prognosis. Any information would be greatly
>> appreciated. Thanks in advance.
>> Jackie Saleh
>> Staff Scientist I
>> Clinical Laboratory Science
>> Genzyme Corporation
>> 1 Mountain Road
>> Framingham,Ma 01701
>> jackie.sa...@genzyme.com <mailto:jackie.sa...@genzyme.com>
>> (508) 271-2972
>> The Institute of Cancer Research: Royal Cancer Hospital, a
>> charitable Company Limited
> by
>> Guarantee, Registered in England under Company No. 534147 with its
>> Registered Office at
>> 123 Old Brompton Road, London SW7 3RP.
>> This e-mail message is confidential and for use by the addressee
>> only. If the
> message is
>> received by anyone other than the addressee, please return the
>> message to the sender by
>> replying to it and then delete the message from your computer and
>> network.

Ryan Farmer

TreeStar Inc.

340 A St. Suite 301
Ashland, OR. 97520

far...@treestar.com
(541) 201-0022 ext. 172
www.treestar.com

www.flowjo.com

www.flowdx.com

www.mycyte.org

Received on Tue Apr 15 13:58:00 2008
This message: [ Message body ]

Flow Cytometry on the Web
MyCyte.org. MyCyte.org ... Purdue. Purdue University Cytometry
Laboratories ... Added MyCyte.org. 2007-07-10 - Corrected Albert
Einstein contact : ...flowcyt.salk.edu/sitelink.html - 54k - Cached

Flow Cytometry Links - Partners AIDS Research Center
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm for the
message
board ... www.mycyte.org A flow website sponsored by Treestar (makers
of Flowjo) ...www.massgeneral.org/aids/Flow Web Site links.asp -
Cached

MyCyte
MyCyte is a web portal for the flow cytometry community. ...
Purdue Cytometry List. Western New York Flow Users Group. Asia.
Australasian Flow Cytometry Group ...mycyte.org - 106k - Cached

MyCyte
MyCyte is a web portal for the flow cytometry community., Swiss
Commission declares HIV patients non-infectious, Purdue adds to the
world of flow cytometrymycyte.org/component/.../Itemid,1/limit,5/
limitstart,65 - 109k - Cached

· Purdue Cytometry Mailing List: Re: clinical flow cytometry

From : Ryan Farmer < far...@treestar.com > Date : Mon Apr 14 2008 -
19:05:51 EDT There is another site of interest along these lines.
Mycyte.org is well established and has a ...

www.cyto.purdue.edu/hmarchiv/Current/0850.htm

Flow Cytometry (FACS) Analysis Software - Life Science
Products ...
MyCyte - PolyChromatic Plot - Compensation Wizard - Daily
Dongle ... http://www.flowjo.com ... Purdue Cytometry Mailing List:
Flow analysis software. Flow ...accuratebiogene.com/search/Flow-
Cytometry-(FACS)-Analysis-Software/...

MailBucket: purdue

MailBucket

· Metroflow meeting July 1

· SAVE THE DATE· Metroflow Meeting· July 1,
2008· · The 2008 Metroflow Spring meeting will be
held on July 1, 2008 at · Princeton University, NJ.·
· This meeting will have a focus on cell life and death, with
clinical and · basic research related talks on apoptosis, the
cell cycle,· cell proliferation and autophagy.· The
technical tools, software, and reagents to study and understand
· these life and death processes will be discussed.·
· A complete speaker list and talk titles as well as
registration · information posted soon on the·
Metroflow web site:· www.metroflow.org· ·
Confirmed speakers include:· · Frank Traganos,
PhD· Zbigneiw Darzynkiewicz, MD, PhD· Kathy Muirhead,
PhD· Jennifer Wilshire, PhD· · ·
See
you there!· · -- · Peter Lopez· Res.
Assistant Professor of Pathology· Co-Director and Manager,
NYUCI Core Cytometry Facility· New York University School of
Medicine· 540 First Ave· Skirball 3 Administration
· New York, NY 10016· 212.263.0635 -- office·
212.263.5907 -- lab· plo...@saturn.med.nyu.edu

ISAC XXII International Congress
FlowJo software. Data showed optimal sensitivity was achieved using
PBMCs ...... CYTOMETRY: PURDUE UNIVERSITY. CYTOMETRY LABORATORIES. J.
Paul Robinson ...
doi.wiley.com/10.1002/cyto.a.20046.abs - Similar pages - Note this
Welcome to Flow Cytometry Core Facility
FlowJo Kinetics Tutorial. ? FlowJo Software Reference Manuals: ...
Purdue flow cytometry. Technical Center for those using secondary and
tertiary ...
www.aecom.yu.edu/facs/ProtocolsLinks.html - 12k - Cached - Similar
pages - Note this
Flow Links - 9 visits - Mar 3

FlowJo Flojo help manual guide lesson help. ... J. Paul Robinson
Gives
WikiPedia an "A" OK, the main Flow Cytometry entry got an A, but Dr.
Robinson felt ...
www.flowjo.com/home/links.html - 22k - Cached - Similar pages - Note
this

Fluorescence Compensation
Jennifer Wilshire, PhD jenni...@flowjo.com MetroFlow Meeting,
Princeton, NJ .... Purdue Cytometry Mailing list - http://www.cyto.purdue.edu.
Practical Flow ...
www.metroflow.org/archive/2004Jan06/abstracts/Jennifer_Wilshire.htm -
39k - Cached - Similar pages - Note this

Purdue Cytometry Mailing List: SV: Thanks for the suggestions -
... cytometry/WEASELv2.html> http://www.wehi.edu.au/cytometry/WEASELv2.html
Winlist 3d from Verity House Software (www.vsh.com) Regards Tim
Timothy Bushnell, Ph.D. Director, CPBR ... Contemporary messages
sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ...
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Purdue Cytometry Mailing List: Re: Thanks for the suggestions -

[Disclaimer: Yes, I live off FlowJo sales, and that's a blatantly
commercial statement, but it gets technical from here on.] We've
played with spatial 3D ...
www.cyto.purdue.edu/hmarchiv/2006/0939.htm

Tim Bushnell & Ryan Duggan ..... Purdue
University Cytometry Laboratories, West Lafayette, IN ...
www.gliifca.org/pdf/GLIIFCA-2004.pdf - Similar pages - Note this

MyCyte

Thanks > > Tim > > > > Timothy Bushnell, Ph.D. > > Research Assistant
Professor, ... Justin, We're working hard to finalize FlowJo
Collectors' Edition, ...
mycyte.org/index.php?

option=com_newsfeeds&task=view&feedid=11&Itemid=70 - 133k - Cached -
Similar pages - Note this

From: Adrian Smith <A.Sm...@centenary.usyd.edu.AU>

(Disclaimer: - I provided a some input into the way the platform
works and I have been beta testing FlowJo for a while now)

ISAC Congress Bilologiacl Councilor, UR, Webmaster and Great lakes
Imaging

Sales Rep?

Subject: Final Call for Flowjo order
From: "Bushnell, Timothy" <tim_bushn...@URMC.ROCHESTER.EDU>
Reply-To: Bushnell, Timothy
Date: Wed, 13 Aug 2008 16:59:51 -0400
Content-Type: multipart/alternative
Parts/Attachments: text/plain (39 lines) , text/html (40 lines)

Content-Type: text/html
This is the final call for the bulk Flowjo order. I need all orders
in by Monday afternoon (8/18). The original message is below:
------------

Hello all:

In case you missed our spring order, I am putting together another
order for Flowjo software. I will be placing it in the month of
August, to try to take advantage of the “Buy 2- get 1 free” sale.

Currently a single dongle costs $1495. However, if there are three
investigators interested in getting a dongle during this promotion,
each dongle would cost $997 (2x1495 = 2990/3 = $997). This is a
great
price. There is also no limit to the number of free dongles
available.

So, if you are interested, please email me the following information:

PI:
Contact name and number:
Account number to charge:

Regards
Tim Bushnell

--
Tim Bushnell, Ph.D.
Co-Director, URMC Flow Cytometry Facility
Office: 585-273-5535
Cell: 585-690-5157
http://www.urmc.rochester.edu/flow-core/
http://www.urmc.rochester.edu/wnyfug/

____UR_Cytometry______Subscribers Corner_____ https://lists.rochester.edu
LISTS.ROCHESTER.EDU ( UR_CYTOMETRY: 24 matches.. )

Item # Date Time Lines Subject
000139 2008-08-13 16:59 112 Final Call for Flowjo order
000138 2008-08-06 14:54 75 Summer Flowjo special
000131 2008-07-17 08:49 104 Re: Reminder: Flowjo lecture and support
000129 2008-07-17 06:24 73 Reminder: Flowjo lecture and support
000127 2008-07-14 14:05 87 Tree Star Flowjo Training/discussions,
Thursday, July 17th
000081 2008-03-14 12:21 228 Reminder: Spring Flowjo order
000079 2008-03-03 13:03 221 Spring Flowjo order
000070 2008-01-23 13:24 291 Feedback requested on Flowjo web seminars
000069 2008-01-18 09:57 249 Reminder: TODAY 1 pm - Flowjo Seminar on
DNA Analysis and Cell Proliferation
000068 2008-01-16 11:31 378 Resend: Reminder: Friday 1 pm - Flowjo
Seminar on DNA Analysis and Cell Proliferation
000067 2008-01-16 09:59 220 Reminder: Friday 1 pm - Flowjo Seminar on
DNA Analysis and Cell Proliferation
000066 2008-01-11 10:31 221 Reminder: Today 1 pm - Flowjo Seminar on
Compensation and Transformation
000065 2008-01-09 11:20 214 Reminder: Friday 1 pm - Flowjo Seminar on
Compensation and Transformation
000063 2008-01-04 10:12 193 Reminder: 1 pm - Flowjo Seminar
000061 2008-01-02 09:46 244 January is Flowjo month at URMC
000057 2007-12-04 09:13 267 January is FlowJo Month at URMC
000047 2007-10-19 10:11 209 Today is the last day to join the Flowjo
bulk order
000046 2007-10-17 10:55 284 Last call - Fall Flowjo order
000042 2007-10-09 09:29 256 2nd call - Fall Flowjo order
000040 2007-10-01 09:30 242 Fall Flowjo order
000034 2007-07-12 06:35 198 Reminder: Flowjo seminar today 1-3 pm
000032 2007-07-03 15:36 88 Post WNYFUG 2007 FlowJo Seminar
000005 2007-04-12 09:41 218 Reminder - Flowjo order
000002 2007-04-03 14:55 248 Flowjo purchase 2007

*********************************************************************************************

Answer: The Purdue Cytometry discussion group is strictly for
discussion of scientific issues relating to the fields in which ISAC
is generally involved. This includes flow cytomery and imaging and
basic science areas of cellular function, cell cycling, immunology,
microbiology and the many areas where single cells or tissues are
analysed.

To join you must send a request to: Subscr...@flowcyt.cyto.purdue.edu
and you must include your name, your institution and your area of
scientific interest. All subscriptions are reviewed - it is not
automatic.

**********************************************************************************************

> From: J. Paul Robinson
> Reply To: j...@flowcyt.cyto.purdue.edu
> Sent: Friday, August 20, 2004 2:25 PM
> To: Cytometry Mailing List
> Subject: Re: mr on Apple web site

> ummm....Mario says..
> "In life sciences - particularly in research life sciences -
> probably 50 to 70% of research laboratories used
> Macs"....while I have a passionate dislike for Windows......is
> this really true ??? or is the key word there "used"?? (Ok...I
> have put on my helmet and armor....waiting...)
> paul

> > For all of the mr groupies out there in cytometry cyberspace. Don't wet
> your
> > pocket protectors over this.

> > Honestly though, well deserved praise for Mario & the Tree Star group:
> > http://www.apple.com/science/profiles/roederer
**********************************************************************************************

Re: mr on Apple web site

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From: Howard Shapiro <h...@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST

Robin Barclay wrote:
>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
>write programs in any language (especially for accessing/controlling lab
>apparatus) - back in the '70's when there were several different options -
>not just PC's. I am glad that the "PC" became a standard (there were too
>many diferent systems) and that IBM did not hang on to it the way Apple kept
>the Mac in house (you get more for your money with a PC because many
>different people make them). You don't often get PC people knocking Macs
>the way that the Mac people knock PCs - there seems to be a lot of Microsoft
>paranoia. In my opinion PCs are much more common and versatile in labs than
>Macs - especially outside the USA - and will eventually become the standard
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.

I can't pass up the opportunity to get into the PC/Mac battle - I hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
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Re: mr on Apple web site
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From: Howard Shapiro <h...@shapirolab.com <mailto:h...@shapirolab.com?
subject=Re:%20mr%20on%20Apple%20web%20site>>
Date: Wed Aug 25 2004 - 18:15:38 EST

-Howard
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RE: mr on Apple web site
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From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,

I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we have
MACs
(and associated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.

Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.

Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.

Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov

> ----------
> From: J. Paul Robinson
> Reply To: j...@flowcyt.cyto.purdue.edu
> Sent: Friday, August 20, 2004 2:25 PM
> To: Cytometry Mailing List
> Subject: Re: mr on Apple web site

> > For all of the mr groupies out there in cytometry cyberspace. Don't wet
> your
> > pocket protectors over this.

> > Honestly though, well deserved praise for Mario & the Tree Star group:
> > http://www.apple.com/science/profiles/roederer
> > > _______________________
> > > Calman Prussin
> > > Laboratory of Allergic Diseases
> > > NIAID/ National Institutes of Health

> > The information in this e-mail and any of its attachments is
> confidential
> > and may contain sensitive information. It should not be used by
> anyone who
> > is not the original intended recipient. If you have received this
> e-mail in
> > error please inform the sender and delete it from your mailbox or any
> other
> > storage devices. The National Institute of Allergy and Infectious
> Diseases
> > (NIAID) shall not accept liability for any statements that are the
> senders own
> > and not expressly made on behalf of the NIAID by one of its
> representatives.

> J.Paul Robinson, PhD PH:(765)4940757
> Professor of Immunopharmacology
> Professor of Biomedical Engineering
> Purdue University FAX:(765)4940517
> EMAIL:j...@flowcyt.cyto.purdue.edu
> WEB: http://www.cyto.purdue.edu

> Have you seen our new HCS webpage?
> http://www.cyto.purdue.edu/hcs

Received on Tue Aug 24 16:18:00 2004
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*************************************************************************
J PAUL ROBINSON

DON'T WET YOUR POCKET PROTECTORS

Re: mr on Apple web site

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From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Mon Aug 23 2004 - 18:46:12 EST
I knew that I should not have taken Mario's bait....but its
been a long summer.....!
paul

> Paul:

> I use Macs exclusively. Between home and labs, I have 7.
> I hate Windows (I tell everyone, I don't do Windows).
> Macs are what Windows wishes it could do. OS X is rock-solid,
> especially Jaguar (OS 10.3).
> BD guys-get with it and release CellQuest Pro for OS X!!!!!!!!!!!!!
> Oh, and Macs are way more photogenic. They look especially cool next
> to your flow cytometer. I just bought a new dual-boot G4 with
> flat-panel display for my flow.
> Beverly

> Beverly E. Barton, Ph.D.
> Assistant Professor
> Department of Surgery/Division of Urology
> UMDNJ-NJMS MSB G519
> 185 S. Orange Avenue
> Newark, New Jersey 07103

> Telephone 973-972-0662
> E-mail barto...@umdnj.edu
> Telefacsimile 973-972-3892

> On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:

> > ummm....Mario says..
> > "In life sciences -- particularly in research life sciences --
> > probably 50 to 70% of research laboratories used
> > Macs"....while I have a passionate dislike for Windows......is
> > this really true ??? or is the key word there "used"?? (Ok...I
> > have put on my helmet and armor....waiting...)
> > paul

> >> For all of the mr groupies out there in cytometry cyberspace. Don't
> >> wet your
> >> pocket protectors over this.

> >> Honestly though, well deserved praise for Mario & the Tree Star group:
> >> http://www.apple.com/science/profiles/roederer
> >>> _______________________
> >>> Calman Prussin
> >>> Laboratory of Allergic Diseases
> >>> NIAID/ National Institutes of Health

> >> The information in this e-mail and any of its attachments is
> >> confidential
> >> and may contain sensitive information. It should not be used by
> >> anyone who
> >> is not the original intended recipient. If you have received this
> >> e-mail in
> >> error please inform the sender and delete it from your mailbox or any
> >> other
> >> storage devices. The National Institute of Allergy and Infectious
> >> Diseases
> >> (NIAID) shall not accept liability for any statements that are the
> >> senders own
> >> and not expressly made on behalf of the NIAID by one of its
> >> representatives.

> > J.Paul Robinson, PhD PH:(765)4940757
> > Professor of Immunopharmacology
> > Professor of Biomedical Engineering
> > Purdue University FAX:(765)4940517
> > EMAIL:j...@flowcyt.cyto.purdue.edu
> > WEB: http://www.cyto.purdue.edu

> > Have you seen our new HCS webpage?
> > http://www.cyto.purdue.edu/hcs

J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:j...@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu

Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs
Received on Tue Aug 24 15:58:00 2004
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Flow Links - 9 visits - Mar 3
FlowJo Flojo help manual guide lesson help. ... in Flow Cytometry -
Los Alamos. Purdue University Cytometry Laboratories * 2005 Mail
List,
Thread Sort ...
www.flowjo.com/home/links.html - 22k - Cached - Similar pages - Note
this

Purdue Cytometry Mailing List: RE: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... We are
currently working on adding the support of these files in the Windows

Ø version of FlowJo, ...

www.cyto.purdue.edu/hmarchiv/2004/1020.htm - 9k - Cached - Similar
pages - Note this

Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
www.cyto.purdue.edu/hmarchiv/2004/0997.htm - 8k - Cached - Similar
pages - Note this

The Science Advisory Board - Protocols, Product Reviews, Member ... -
48 visits - Mar 3
Flow Cytometry: A Brief Introduction and Guide to On-Line
Resources ... Dr. J. Paul Robinson, Purdue University School of
Veterinary Medicine, ...
www.scienceboard.net/community/perspectives.169.html - 30k - Cached -
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Beverly E. Barton, Ph.D.? LOVES THE LAB? IT TOOK ME 30 SECONDS

Data and Image Analysis Special Interest Group Meeting 2007J. Paul
Robinson, SVM Professor of Cytomics, Purdue University and
President, ... (*) Robert C. Leif, Newport Instruments. "Cytometry
Standards Continuum" ...
www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages -
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The Science Advisory Board - Protocols, Product Reviews,
Member ...Flow cytometry started as a simple instrument designed
during World War II to ... Dr. J. Paul Robinson, Purdue University
School of Veterinary Medicine, ...
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Flow cytometer analysis on PC
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Author Topic
mlinde
Member
United States
11 Posts Posted - February 03 2006 : 6:21:13 PM
________________________________________
I'm looking into flow cytometer analysis programs for the PC and I
was
wondering whether anyone had any insight into which programs were the
most useful and worthwhile. From what I have seen, Flow-Jo and
FCSexpress are the more commonly used PC programs. Does anyone have
any experience with either of these programs? The other option would
be to purchase another Macintosh and use CellQuest. Advice, comments,
suggestions?
rakeshverma
Member
United States
93 Posts Posted - February 04 2006 : 10:46:54 PM
________________________________________
I have used CellQuest. Its a good option.
Antonio68
Member
Germany
20 Posts Posted - May 01 2006 : 11:48:43 AM
________________________________________
You can use Summit from Dako is free and gives you the possibility to
do offline compensation. However, the best flow cytometry software is
FlowJo. There is a PC (Java based) and a Mac version. The Mac version
for the moment is quite better.
lovesthelab
Senior Member
United States
804 Posts Posted - July 06 2006 : 4:59:46 PM
________________________________________
I'm not a PC person, but am an ardent flow person. My flow friends
with PCs swear by WinMidi, which is free. If you Google for free flow
cytometry software, you'll come to 2 sites, one at UMass, one at
Scripps. Lots of free analysis software for the PC, maybe because BD
relied on Macs for so long.
mlinde
Member
United States
11 Posts Posted - August 02 2006 : 5:44:55 PM
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy with either of them compared to Cell Quest. I
found FCSexpress almost impossible to work with. FlowJo was alright,
but I don't think I had the time to really get the software down.
Anyone know if BD ever plans to put out CellQuest on PC?
lovesthelab
Senior Member
United States
804 Posts Posted - August 09 2006 : 1:22:04 PM
________________________________________
quote:
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy wi