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ISAC Congress San Diego Meeting

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All this marketing crap

just gets in the way of my programming.

 

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:35:13 EST

RE: Report from ISAC Meeting San Diego

From: Adam Treister (adam@treestar.com)
Date: Wed May 22 2002 - 01:45:52 EST


> From: J.Paul Robinson [mailto:jpr@flowcyt.cyto.purdue.edu]

 

> Subject: Report from ISAC Meeting San Diego    

 

> Colleagues:

 

> Hello from sunny California and the ISAC XXI congress. This message comes

to you

 

> from the CYBER CAFE generously provided by

 

Adam Triester of Tree Star, Inc of FLOJO land!

 

 

> Adam has made a bank of 12 computers, a wireless network and, lots of

network cables for

>  laptops. He has a T1 fast line and is providing FREE access for the

entire congress.

 

> The room is ALWAYS full and is definitley the most popular place in the

congress.

 

> It has nothing to do with the outstanding FREE coffee from Ryan Bros,

Coffee again

 

> generously provided by Adam.

 

 This is the best facility provided by any vendor ever!.....

 

> so long live FLOJO.....

 

and more free coffee and internet access....

 

Paul,

 

 

Thanks for the kind words, but I can't take all the credit for the

CyberCafe.

 

Apple generously provided all the Macs. 

 

IT departments around the world may

say that Macs are hard to network, but we put a dozen Macs on the Internet

in under an hour.   Apple sent top of the line G4s with Cinema displays, and

Titanium PowerBooks, as well as a bunch of iMacs.  Imagine what the lines

would have been like if we only had three computers, as we did in

Montpelier.

 

The true highlight of the cafe was the free espresso. Thanks to Phoenix Flow

Systems, Guava Technologies, PROzyme and Becton Dickinson for contributing

to the coffee fund. By the time we got to the coffee

 

I had blown the marketing budget on this endeavor,

 

and these companies stepped in to make

sure you had Ryan Brothers coffee instead of the swill we'd have gotten from

the hotel. 

 

I also extend a special thanks to Kevin Becker for bringing the

Mar Dels to the banquet. 

 

The best band of any ISAC I've attended.

                                

The CyberCafe crew was Jennifer Wilshire, Maciej Simm, Adam Treat and Amy

Hsu.

 

They thought they were getting a leisurely week on the beach, and ended up working 9 to 9 every day to keep the CyberCafe running. 

 

It

was an

exhausting schedule, and they were tireless in their support of the

attendees' Internet needs.

 

It never would have come off without them.

 

Sophia Ascani and Alexandra Treister tie-dyed the 350 shirts.  Each one is a

unique work of art, and each was hand-dipped.  Our garage floor has the

stains to prove it.  So wear them proud, and wash them in cold water.

 

We've agreed to do it again at ISAC XXII in Montpelier. 

 

I'm just thankful this

 

congress is only held every second year. 

 

All this marketing crap

 

just gets in the way of my programming.

 

Au revoir,

 

Adam

------------------------------------------------------------------

Adam Treister

Tree Star, Inc.

ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186

adam@treestar.com    www.flowjo.com

------------------------------------------------------------------



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flowcytometry said:

Just one more stupidity by a manufacturer

From: Mario Roederer (ROEDERER@Darwin.Stanford.EDU)

Date: Tue Jun 28 1994 - 16:59:16 EST

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________________________________________

I just wanted to relate a little story... draw from it your own

conclusions.

Here at Stanford we were enthusiastic about one of BD's new software

products, Attractors.  It would be obviously useful to our ongoing

clinical trial...  About 6 months ago, I went to BD to test the software

with some of our data (collected here, using our own software), and

it worked quite well.

About 2 weeks ago, a BD salesperson stopped by with a demo version,

trying to sell us on the software.  We decided to go ahead and test it

on our data.  Much to my surprise, it could not read our data files!  

(It gave a generic file reading error, stating that the file was damaged

or not an FCS file.)  I called up BD and spoke with one of the programmers.  

I was informed that the decision had been made by "BD Sales" to modify the

FCS header reading portion of Attractors so as to only read FCS files

created by BD's software (i.e., HP-generated)!  (The reasoning being,

apparently,that they didn't want people with Coulters to be able to use the

program.)

So much for our demo.  And so much for the possibility that we will be

purchasing Attractors, since we don't generate data with BD software.  

(Hey sales people at BD:  do you know how many macintoshes there are

here at Stanford that could be using your software to analyze data?  50?

100?)  And it also means that the market for Attractors is significantly

limited.  Is this really good "Sales" policy?

In the open-computing environment that is the general trend of this

decade, this kind of decision is rather insulting to the user community.

Mario Roederer

(PS--the sale pricing on Attractors was also interesting:  as quoted to

us, it is cheaper to buy a total of 6 copies than to buy 3.  Go figure.)

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Re-Attractors Software Technology

From: Mike Lance (Mike_Lance@bdis.com)

Date: Wed Jun 29 1994 - 11:29:26 EST

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________________________________________

                      Subject:                               Time:4:22 PM

 OFFICE MEMO          Re: BD Attractors Software Technology  Date:6/29/94

Re: BD Attractors Software Technology

In reaction to Dr. RoedererUs message about file compatibility of  BDUs

recently introduced Attractors software technology, we would like to make

the following comments:

BDIS invested in the development of this unique software tool to enhance the

value of its Instrument and Reagent systems. It was decided to make the first

release of the software compatible with list mode data files that are

generated by BD CONSORT 30 and 32, as well as by BD FACStation data

management systems. This being consistent with our general Systems approach.

The product literature clearly documents the supported compatibilities.

In addition, we would like to point out that to our knowledge, none of the

analysis software of the major cytometer manufacturers claim to support

files generated from other systems.

As to pricing, we use a common volume-based pricing policy, whereby

additional copies of the software cost the end user less than the first.  6

copies in this policy are not less expensive than 3.

Johan G. M. Christiaanse

Group Marketing Manager

Becton Dickinson Immunocytometry Systems

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Re: Re- attractors software NONcompatibilities

From: Robb Habbersett (robb@beatrice.LANL.GOV)

Date: Wed Jun 29 1994 - 21:28:12 EST

• Next message: J. Paul Robinson: "Will 'attractors' be attractive?"

• Previous message: Mike Lance: "Re-"

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________________________________________

The Flow Cytometry Standard (FCS data file format) just became even

less of a standard!  Endorsed out of one side of the manufacturer's

mouths, while out of the other side, the boys in sales offer the

latest pitch!  In reality, an open standard - truely supported by all

the major players - would be in everyone's best interest, in the long

run as well as the short.  Here's a radical concept - the folks with

machines from the COMPETITOR think the Attractors software is really

neat and they decide to ... you guessed it - they buy a copy!!  WOW.

Robb Habbersett

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Re: Becton Dickinson Attractors Software

From: Harbor Branch Oceanographic Institution (harbolon@CLASS.ORG)

Date: Thu Jun 30 1994 - 17:57:50 EST

• Next message: Alice L. Givan: "printers"

• Previous message: Luther K. Barden: "RE: Becton Dickinson Attractors Software"

• In reply to: BOB FEINBERG: "Becton Dickinson Attractors Software"

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________________________________________

Bob:  I think you have it wrong!  You said that the new Attractors

software is the "engine" that drives the hardware and why buy a Chevrolet

and put a BMW engine in it when you can buy a BMW.  I think you have that

backwards!  BD's hardware is the Chevrolet with the BMW engine and

Coulter's hardware is the BMW with the Chevrolet engine (software).  BD

has always had the "flashy software" (albeit incompatible with DOS).  

It's too bad that BD can't figure out that they could make some extra

bucks with some "cross-platform" software--duhh--there are companies out

there that are making it with such an idea.  Why don't you guys wise up

and take the plunge and quit hiding behind your software--us Mac guys

will figure it out anyhow, regardless of what hardware we run!

Meanwhile, I will drive my BMW (Coulter) with the underpowered software

engine.  Who knows?  I might decide to drop a Porche engine in under the

hood.  Now that would be a combination!  At least I'll know that the

doors will stay on and it won't fly apart like the Chevy!

Ross E. Longley, Ph.D.   *   Harbor Branch Oceanographic Inst., Inc.

Immunology Group Leader  *   5600 U.S. Highway #1, North

Div. Biomed. Marine Res. *   Fort Pierce, FL  34946

Phone (407) 465-2400, 486

FAX (407) 465-1523

e-Mail harbolon@class.org

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

RE: Becton Dickinson Attractors Software

From: Luther K. Barden (Luther_Barden@nih.gov)

Date: Thu Jun 30 1994 - 18:04:08 EST

• Next message: Harbor Branch Oceanographic Institution: "Re: Becton Dickinson Attractors Software"

• Previous message: Luther K. Barden: "RE: Attractors"

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________________________________________

In reference to Bob Feinberg's latest posting...

> Software such as Attractors is the engine that drives our

> hardware.  Would you purchase a Chevrolet and replace the

> engine with a BMW's?

> If you want the performance of the BMW, you buy a BMW!

<flame on>

Some of us are used to jacking up things like a FACS-II (with the PHA,

remember) and sliding in a four channel ADC box, an LSI-11, a PDP-11/34,

and a raft of in-house acquisition and analysis software and showing the

world what can be done with a properly instrumented cytometer.

... which is more like installing a Ferrari engine in a VW Bug, and is a

lot of fun when you finally get it to work!

<flame off>

... Reality Check ...

We do need to keep a bit of perspective here.

The emphasis on the importance of analysis software is relatively new at

BDIS, and should perhaps be encouraged (;-).

Crippling (or licensing) a software product so it won't work with your

competitor's data files is no great crime.  It just limits the market for

the software, and encourages third party developers to come up with

something truly general.

Are you listening, Verity ??

So long as the acquisition system's data files are well defined (hopefully

even compliant with published standards) we'll be able to cope.

... Luther

________________________________________

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________________________________________

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Will 'attractors' be attractive?

From: J. Paul Robinson (paul)

Date: Thu Jun 30 1994 - 10:09:59 EST

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________________________________________

A comment on the attractors software from BD.

The issue of manufacturers making their software totally compatible with

every flow file format has been discussed ad nauseam for donkey's years

to little avail. The fact is that they create markets to sell their products.

The bottom line is that someone will write a conversion program fairly

quickly (Phoenix or Verity software or Steve kelly or someone!!) that will let

you take advantage of these programs.

The use of fairly smart programs to evaluate immunophenotying data is not new,

but it has been getting more attention, particularly since one of the big

flow companies (B-D) has paid some attention to this area.

Don Frankle and myself are chairing the "Data Acquisition, Processing and

Analysis" workshop at ISAC and you might be interested that a paper on

attractors from teh B-D group will be presented entitled

-

"Cellular multiparameter cluster analysis using hierarchical attractors" by

P.Bierre.

We would be happy to discuss the issus in the workshop, which is one of the

functions of the workshop I guess.

Paul Robinson

Purdue University Cytometry Laboratories

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Becton Dickinson Attractors Software

From: BOB FEINBERG (73404.2032@compuserve.com)

Date: Thu Jun 30 1994 - 11:16:50 EST

• Next message: Mario Roederer: "FCS data standards"

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• Reply: Harbor Branch Oceanographic Institution: "Re: Becton Dickinson Attractors Software"

• Maybe reply: Eric Martz: "RE: Becton Dickinson Attractors Software"

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________________________________________

Thank you for all your comments about Becton Dickinsons' sales

strategies.  Becton Dickinson is in the "business" of selling flow

cytometry instrumentation.  Attractors is a new exciting software

product that BDIS developed at substantial cost and development time.

 Why should the products that we develop offer

any advantage to our competitors?  BDIS has shown and provided tremendous

support to the research industry over the years, and has been more than happy

to share information with our loyal customers and potential customers.

Software such as Attractors is the engine that drives our hardware.  Would

you purchase a Chevrolet and replace the engine with a BMW's?

If you want the performance of the BMW, you buy a BMW!

For those who have chosen to purchase our competitors products and now want what BD  

has to offer, I'm sure your local instrument specialist would be more than happy

to discuss a trade-up program with you.

Robert Feinberg

Instrument Sales Manager

Central Region

Becton Dickinson Immunocytometry Systems

________________________________________

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• Reply: Harbor Branch Oceanographic Institution: "Re: Becton Dickinson Attractors Software"

• Maybe reply: Eric Martz: "RE: Becton Dickinson Attractors Software"

• Maybe reply: Susan D. DEMAGGIO: "Re: Becton Dickinson Attractors Software"

• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

FCS data standards

From: Mario Roederer (ROEDERER@Darwin.Stanford.EDU)

Date: Thu Jun 30 1994 - 12:38:53 EST

• Next message: James W. Jacobberger: "BD, Attractors, FCS file format, etc."

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________________________________________

I agree with Paul about saying too much about supporting all data

types...  There should be a minimal FCS configuration that ideally will

be supported by any program.  But to stave off a lengthy EMail

discussion about data standards, I want to clarify that the problem

with Attractors is not one of a data standard.

Indeed, Attractors does support the FCS standard just fine.  As pointed

out by BDIS in their mail message, it reads Consort 32 files, etc.  

This obviously means that it simply checks the FCS header keywords for

particular configuration of values specifying original acquisition on BD

software.  (er, I mean, "Current" BD acquisition software, since

Consort/VAX isn't included).  However, if a file does not have this

configuration, Attractors gives a very misleading error message:

"This file is either damaged, or not an FCS file"

rather than telling you that it won't analyze "foreign" FCS files.  If

BDIS is so open about documenting compatibility, why is this error

message so obscure?

Mario

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

BD, Attractors, FCS file format, etc.

From: James W. Jacobberger (jwj@po.CWRU.Edu)

Date: Thu Jun 30 1994 - 13:47:30 EST

• Next message: Gary C. Salzman: "Attractors"

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________________________________________

My thanks to BD and all initiators, repsonders, and Attractors

for a small but lively segment of an otherwise unbearable

afternoon.

Jake

--

James W. Jacobberger

Case Western Reserve Univ.

216-368-4645 (voice)

216-368-3432 (FAX)

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Attractors

From: Gary C. Salzman (Gary_Salzman@lanl.gov)

Date: Fri Jul 01 1994 - 02:22:40 EST

• Next message: Becky Bonner: "Re: Fixation prior to staining"

• Previous message: James W. Jacobberger: "BD, Attractors, FCS file format, etc."

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________________________________________

       I don't believe that BD has messed with the FCS standard.  What

they have done is to use the resource fork of the Mac file to identify the

file type that Attractors will read and the file type that it will write.

Attractors will read files of type 'BDLM' and writes a type of 'AttR'.  If

you have the Apple ResEdit (resource editor), you can edit the resource

fork of your Mac file to change the type from '????' to 'BDLM' so that

Attractors will read it.  This editing consists only of changing a single

entry in a simple dialog box.  If you are writing Mac software, it is a

simple matter to set a constant so that you can open any type of file.

Best, Gary

Gary C. Salzman

Life Sciences Division

Los Alamos National Laboratory

Mail Stop M888

Los Alamos, New Mexico 87545

voice: (505) 667-5503

FAX:  (505)665-3024

email: Gary_Salzman@lanl.gov

________________________________________

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

Attractors

From: Eric Martz (emartz@microbio.umass.edu)

Date: Fri Jul 01 1994 - 14:34:22 EST

• Next message: Eric Martz: "RE: Becton Dickinson Attractors Software"

• Previous message: Dennis Sasaki: "Seeking Flow Cytometry Position"

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________________________________________

Would someone please give a brief explanation of what Attractors software

from B-D does, for the enlightenment of many on the cytometry email list?

/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Eric Martz, Professor of Immunology          emartz@microbio.umass.edu

Dept Microbiology              Voice: 413-545-2325   FAX: 413-545-1578

Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/

________________________________________

• Next message: Eric Martz: "RE: Becton Dickinson Attractors Software"

• Previous message: Dennis Sasaki: "Seeking Flow Cytometry Position"

• Next in thread: Luther K. Barden: "RE: Attractors"

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________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

RE: Becton Dickinson Attractors Software

From: Eric Martz (emartz@microbio.umass.edu)

Date: Fri Jul 01 1994 - 15:07:23 EST

• Next message: Eric Martz: "Re: Color printers"

• Previous message: Eric Martz: "Attractors"

• Maybe in reply to: BOB FEINBERG: "Becton Dickinson Attractors Software"

• Next in thread: Susan D. DEMAGGIO: "Re: Becton Dickinson Attractors Software"

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________________________________________

In message Thu, 30 Jun 1994 19:04:08 -0400,

 Luther_Barden@nih.gov (Luther K. Barden)  writes:

> Crippling (or licensing) a software product so it won't work with your

> competitor's data files is no great crime.  It just limits the market for

> the software, and encourages third party developers to come up with

> something truly general.

It also encourages those of us who have written 'truly general' analysis

software to meet our in-house needs to make it freely available, e.g.

my MFI, Joe Trotter's WinMDI, and Rob Habbersett's IDYLK.

/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Eric Martz, Professor of Immunology          emartz@microbio.umass.edu

Dept Microbiology              Voice: 413-545-2325   FAX: 413-545-1578

Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/

________________________________________

• Next message: Eric Martz: "Re: Color printers"

• Previous message: Eric Martz: "Attractors"

• Maybe in reply to: BOB FEINBERG: "Becton Dickinson Attractors Software"

• Next in thread: Susan D. DEMAGGIO: "Re: Becton Dickinson Attractors Software"

• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

________________________________________

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST

The Attractor Controversy / Printers

From: Howard Shapiro (HPANDA@HARVARDA.HARVARD.EDU)

Date: Mon Jul 04 1994 - 14:56:51 EST

• Next message: Birger Christensson: "Cytoron-Absolute"

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________________________________________

It was nice to find both thermal (attractors) and inkjet (printers)

discussions on the net after I got back from a two-week cruise...I had

a computer with modem along, but elected not to pick up my E-mail via

satellite at $11/minute.

The attractors debate seems to indicate that FCS is not a standard.  I

know it's supposed to be a flexible standard, and that, in principle,

programs can be written to parse and decipher anything which falls under

the spec, but that's not what's happening in the real world.  If Verity

or Phoenix or somebody else has to write a conversion program to convert one

flavor of FCS to another, what's the point?  And, if such a program does get

written, are manufacturers going to be diddling other file characteristics

such as resource fork information to make programs choke on data not

acquired with their instruments?  Never mind the translation program; what

will happen is that somebody will write a freeware program which does what

Attractors does and reads any FCS format within reason.

The "BMW / Chevrolet" model speaks to the major segment of the flow cyto-

metry market which sticks with off-the-shelf hardware, reagents, and

software.  Even in that market segment, instrument manufacturers who also

make antibodies are facing stiff competition from third parties with

cheaper reagents.  It's entirely natural for a manufacturer to try to lock

the advantages of a new product in to its own user base.  Those of us who

do customize or build our instruments are at a disadvantage as a result,

but the manufacturers, who stand to make far less money from us than from

the much larger community of less sophisticated users, don't have much

incentive to care.  The moral is that if you're prepared to do your own

hardware, you'd better be prepared to do your own software.

The printer manufacturers aren't doing any better by the flow cytometry

community than are the instrument manufacturers.  The present crop of

color printers (Canon BJC-600 and Apple's Color StyleWriter Pro, which use

Canon's 360 x 360 DPI engine, and H-P's DeskJet 560C and DeskWriter 560C,

which use H-P's "600"x300 DPI engine) are oriented toward producing more

realistic printouts of scanned color photographs than you could get with

those manufacturers' older 180 DPI color printers.  In my experience,

neither the new Canon nor the new H-P printers are as reliable as the old

Canon A-1210 and PJ-1080 (and Quadram, IBM, and Radio Shack Printers which

used the same engine) or H-P PaintJet.  When running under Windows, or

under the Mac Operating System, both the Canon and H-P printers are extremely

slow; for Windows users, SuperPrint, from Zenographics, provides somewhat

faster operation.  You can also spend up to $10,000 for color printers using

thermal wax transfer, dye sublimation, and dry ink jet technology, but what

you'll get is better color saturation and, sometimes, PostScript; the big

expensive printers may be even slower than the smaller ones.  I've been

thinking of trying to get a license from H-P to manufacture PaintJets.  It

seems that the whole scientific instrument market, which can make out fine

with 180 DPI printers, isn't big enough for H-P or Canon to care about.

Everybody's watching his/her bottom line; this is mine.  --Howard

________________________________________

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(no subject)BD’s Attractors Software

From: Thomas Delohery (t-delohery@ski.mskcc.org)

Date: Tue Jul 19 1994 - 23:58:33 EST

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________________________________________

For me, the recent discussion regarding BD's "Attractors" software

was very entertaining if not informative.  One of the more interesting

things to come out was the fact that BD doesn't consider ConsortVAX

to be a "current" acquisition software.  That being the case, I'm calling

on BD to do the research community a service and release ConsortVAX

to the public domain.  The author of CVAX, Bob Murphy, has urged BD

to release it to no avail.

At least the analysis portions of CVAX (disp4(8), disp2d, hidden, cotfit)

could be released in the public domain with a disclaimer by BD saying the

software is "unsupported" and they would only be responsible for

"supporting" CVAX that people have purchased from BD.  

The argumentative tone of the "Attractors" discussion shows there is a lot

of frustration among the flow community (at least the research folks) with

available software.  CVAX should be added to WinMDI, MFI and IDLYK

as freeware in the public domain so that people with the ability and inclination

to improve or adapt the programs to their needs can do so.  

I remember a couple of  years ago on this bulletin board someone requesting

Bob to release the CVAX code in the public domain (Leon? in Australia?).

If others are interested, I suggest they post something here as it's obvious

that BD is listening.

tom d.

--

==============================================================================

Tom Delohery                           | Internet: t-delohery@ski.mskcc.org

Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729

Memorial Sloan-Kettering Cancer Center |      Fax: (212) 794-4019

==============================================================================

________________________________________

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BD Attractors Overview

From: Pierre Bierre (Pierre_Bierre@bdis.com)

Date: Tue Jul 26 1994 - 11:01:50 EST

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________________________________________

                      Subject:                               Time:3:56 PM

 OFFICE MEMO          BD Attractors Overview                 Date:7/26/94

In response to Eric Martz who asked what BD Attractors software does:

Attractors is a new data analysis automation package available for off-line

analysis of FACS-acquired listmode data files on the Macintosh.

An "attractor" is a dynamic population gate, defined by the user, which

adapts positionally to tube-to-tube variations in the location of its

associated data cluster using a gravitational attraction algorithm.   A set

of attractors, whereby one attractor has been defined for each population of

interest, comprises a complete analysis protocol (called an attractor set).

In general, an attractor set is reusable across tubes prepared under the

same staining protocol.

Attractors is optimized for multiparameter subset analysis (up to

8-parameter data).  Populations may be defined hierarchically, i.e.

sub-populations of sub-populations reaching down several levels into a

compartment.  Each staining dimension naturally affords another level of

population subsetting.  Population statistics are drawn on all populations

and sub-populations the user cares to define. Percentages are automatically

formed relating each subset to all its enclosing supersets.

Visualization features include histogram, 2D dotplot, mouse-rotatable 3D

dotplot, and a new Multi-VU plot useful for viewing higher-order spaces (3D

-> 8D) (yet another try at this folks!).  

Attractors offers a new approach to batch data analysis for multi-tube

panels which does not involve MouseTracks and which does offer flexible

automation.  A batch protocol consists of sequencing a list of attractor

sets, then specifying a folder containing all the listmode files.  Since the

attractor sets are each editable modules, tube-level changes can be

incorporated and batch rerun immediately.  Batch output options include

spreadsheet file generation (tab-delimited ASCII with field name headers)

and auto-print.  Typical analysis speed for Quadra 800 analyzing 10,000

event, 5-parameter data into 6 populations is 220 files/hour.

Pierre Bierre

Research Computer Scientist

Becton Dickinson Immunocytometry Systems

San Jose, CA

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November 26, 2008 3:13 AM
 

flowcytometry said:

EMAIL ABUSE - how to stop

From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)

Date: Thu Dec 12 2002 - 08:43:08 EST

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________________________________________

Colleagues: I am sending out a copy of a message I have just sent to

RNWAY laboratories of South Korea and all 20 worldwide distributors of

RNWAY products most of whom are highly reputable companies. I am only

sending it to you because I am going to propose to create a small

"SCIENTISTS against EMAIL ABUSE"  

type of revolutionary action.........

I won't send you more copies of what I am going to send out,

But

I will post

them in a prominent place on our website so that you can follow and

participate in the action.

I am truly sick and tired of receiving literally dozens of

unsolicited crap  messages each day.

However, receiving them from supposedly reputable companies drives me really crazy.

These, I can do something

about and I am.

The rest are virtually beyoned any reasonable solution. As you

can see, I have

started with RNWAY, a company that I have requested a dozen

times to

remove me from their abusive lists. This week they sent

me an attachment to boot....

- something they do constantly. This made me really mad.

Perhaps it was the beef I ate in the UK this week, but

I got

really mad when RNWAY filled my box yet again.....

and I am going to set up a place where

people can register copies of messages they send recording their

requests

for removal.

This record will become a documentation place you

can copy

your messages to for any future legal actions.  We will code the

email

address of the requestor so you don't get further abuse, but we

will publish

the list of companies that continue to abuse us.

If you have suggestions, please let me know.

Sincerely

"Mad Cow" Paul Robinson

Purdue University

---------------------------------------------------------------------------

Here is the email that I sent to RNWAYs 20 worldwide distributors

---------------------------------------------------------------------------------------------

You are receiving this message if you are associated with RNWAY products.

We have continually requested removal from RNWAYS abusive and constant

emails.

It is against Federal law in the United States

to continue to send

unsolicited emails after requests to discontinue are made.

RNWAY posts a

privacy policy that they make no attempt to enforce.  

Our requests have been

documented by the university on numerous occasions.

RNWAY is now liable

in the United States for severe penalties under US law.

As a distributor of this company you may also incur loss of trade, or reputation for the actions we are going to take.

As scientists who buy your products, we are going to exert our own economic power. We will be letting members of our various associated networks that RNWAY will be listed as an EMAIL SPAM ABUSER.

We will be recommending that all of our users do not purchase any of their products if they continue to abuse our email accounts.

As a scientist who uses the INTERNET as a crucial component of my

communication with others,

I am sick of abuse of my email and I have

decided that I am going to do something about it, one company at a time.

This message has been copied to the 20 companies that distribute RNWAY

products around the world.

This is the first of a series of actions. It will also be

copied to 3000 scientists who may have an interest in stopping this type of

abuse.  

It will also be posted on a  public site that had last year over 5 million access.

If you as a distributor want to make a profit on selling (scientists)

products, I suggest you ensure that the comanies you deal with don't abuse

us.

If they do, we won't buy their products and you will lose money.

Do you hear us?

Yours sincerely

J.Paul Robinson, Professor, Purdue University

------------------------------------------------

J.Paul Robinson, PhD             PH:(765)4940757

Professor of Immunopharmacology

Professor of Biomedical Engineering

Purdue University          FAX:(765)4940517

EMAIL:jpr@flowcyt.cyto.purdue.edu

WEB: http://www.cyto.purdue.edu

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November 28, 2008 2:36 AM
 

flowcytometry said:

FlowJo hiring see us at ISAC Congress if your not coming to ISAC you must NOT be Cool and we Don't want to Hire you!

FlowJo hiring, come see us at ISAC

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From: Adam Treister <a@treestar.com>

Date: Sun May 14 2006 - 20:04:57 EDT

Come join the FlowJo team!

We are looking to expand FlowJo's market presence, train a generation  

of scientists in the subtle beauty of high color flow, and solve some  

small subset of the world's problems.  

If you're coming to ISAC XXIII  

in Quebec, and are interested in making the cytometry world a better  

place,

please bring by your CV and talk to us in Booth 301.  

If you don't need a job,

at least come by to get a tie-dyed t-shirt.

If you're not coming to ISAC,

you must not be cool,

and

we don't want  to hire you.

Unless, of course, you are an exceptional flow user,

with good grasp of the theory behind the machine,

know at least ten  

ways to hold down modifier keys

to perform silly FlowJo tricks,

and

want to share your expertise with a growing user community.

Then,  

please send your CV to jobs@treestar.com,

and

we'll evaluate your feeble excuse

for missing this important conference.  

We'll send you a t-shirt if we have any left.

Adam

-------------------------------------

Adam Treister

President

Tree Star, Inc.

340 A Street

Ashland OR 97520

www.flowjo.com

-------------------------------------

Received on Mon May 15 14:18:00 2006

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November 30, 2008 5:07 PM

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