FlowJo Announcements From: Adam Treister (adam@treestar.com) Date: Mon Apr 22 2002 - 00:15:07 EST • Next message: b cotleur: "2-me in culture media:summary" • Previous message: Geert Raes: "Re: b-mercaptoethanol in media" • Next in thread: Mario Roederer: "Job Opening -- Immediate -- Vaccine Research Center" • Reply: Mario Roederer: "Job Opening -- Immediate -- Vaccine Research Center" • Reply: Michael Dustin : "MoFlo vs Vantage" • Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ Only two more weeks until ISAC, that biennial bacchanalia of flower power and fun! So I hope you'll excuse a bunch of blatantly commercial announcements to the list, but endulge me this one time and read on. At the show we'll be releasing FlowJo Version 4. We've got new platforms for overlaying and clustering, we've made it work better across the Internet, added all sorts of new conveniences, and spiffed it up with the new OS X look and feel. What was already the best analysis software in flow cytometry has gotten a whole lot better. At the meeting, Tree Star is proud to be sponsoring the CyberCafe, your link to home and responsibility while you're in carefree San Diego. Check your email, surf the web, download the slides you'll be presenting at ten. A cadre of California companies has contributed to bring in a premier local roaster to satisfy all your latte urges. We hope you’ll all drop by and see what we mean when we say we're committed to Java. The network is going wireless this year. Just pop a 802.11 card in your laptop, and while your neighbor plays solitaire through the keynote, you can be reading e-mail. We're going to open the CyberCafe with the Second Biennial FlowJo Users Group Meeting,. Saturday night May 4 at 8PM. The first user group meeting was cancelled for lack of interest when Dave Novo brought in a case and a half of French wine, so this year we're going to try real hard to assemble to the point where we can see the show of hands on something before we disband in search of alcohol. We'll have a whole bunch of Macs running FlowJo v4 under OS X, and you can bring your own data and get into big arguments about compensation. Be the first to get the newest FlowJo t-shirt. All you Windows fans out there, come by our booth to see FlowJo running on a PC! We’ll be previewing the long awaited Java version of FlowJo. We’re still not ready to release it, but we’ll be giving a peak as to what it is going to do. For those who haven't found it yet, we've unveiled a spanking new FlowJo website. Flowjo.com is chuck full of new content, functionality and spunk. Automated price quotes, online ordering, a FAQ that will guide you to new depths of understanding, and none of that awful yellow on black text. The search engine even works. No ads & cookie-free. Check it out. Specifically, you should check our pricing. Prices are going up on May 10. It has been a number of years since we've changed our prices and with the development of the OSX and PC versions, it¹s time for a leap. I guess there's no such thing as a free launch. Anyway, this may be a great time to buy that FlowJo ten pack you've been thinking about. Licenses purchased before May 10 are entitled to a year of free upgrades, including the 4.0 release. Unleash the flower power! Adam ------------------------------------------------------------------ Adam Treister Tree Star, Inc. ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186 adam@treestar.com www.treestar.com ------------------------------------------------------------------ ________________________________________ •
FlowJo
From: Manfra, Denise (denise.manfra@spcorp.com)
Date: Tue Jun 19 2001 - 09:37:46 EST
Hi:
I wanted some feedback on the FLOW Jo application. I
currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
may expedite my analysis. However, I do not use the same markers all the
time, and in one experiment I may be running more than one stain with
multiple different antibodies. Basically, very seldom do I run the same set
of markers. How does FLOW Jo work for such conditions?? How about plot
analysis and excel: apparently Excell FLOW Jo puts the data directly into
EXcell?? What has been your experience with FLOW Jo?? Any information would
be useful.
denise
***************************************************************
This message and any attachments is solely for the intended recipient. If
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Wed Jun 20 2001 - 01:25:04 EST
> Hi:
>
> I wanted some feedback on the FLOW Jo application. I
>currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
>may expedite my analysis. However, I do not use the same markers all the
>time, and in one experiment I may be running more than one stain with
>multiple different antibodies. Basically, very seldom do I run the same set
>of markers. How does FLOW Jo work for such conditions?? How about plot
>analysis and excel: apparently Excell FLOW Jo puts the data directly into
>EXcell?? What has been your experience with FLOW Jo?? Any information would
>be useful.
>
> denise
I have been using FlowJo since it was released in 1997. I haven't
used CellQuest since I started using FlowJo.
I have analysed thousands of samples with a lot of different stain
combinations and FlowJo has worked very well for this. In FlowJo you
handle multiple stain combinations by grouping all the samples with
the same stain and then applying an analysis to all the samples (and
you can also make sample-specific adjustments very easily).
FlowJo can export statistics for each group and then you simply open
them in Excel. This features has saved me a LOT of time.
I would highly recommend FlowJo. I could go on about its benefits but
you can search the archives for my previous posts on FlowJo or read
the review I wrote at
<http://www.biocompare.com/prorev.asp?profrevid=49> for more of my
opinions. Better still, download the latest version and ask for a
free trial serial number. Make sure you do the tutorial and then test
it out on your own samples.
If you have any specific questions after you have tried it out feel
free to ask.
Adrian Smith
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Giovanna Borsellino (gborsel@tin.it)
Date: Thu Jun 21 2001 - 04:26:47 EST
Hi Denise,
I very highly recommend FlowJo! In our lab we perform
polychromatic flow cytometry routinely: our samples can be stained with up
to 9 different fluorochromes, and we have multiple samples with different
markers which have to be analyzed in each experiment. We have tried several
software packages, and when we found FlowJo we stopped searching. Actually,
now we use FlowJo even for samples stained with 'only' 3 colours, which we
acquire on the FACScan.
The way FlowJo works is slightly different from other flow-cytometry data
analysis software: basically in FlowJo you create "workspaces" where you
store all the information concerning your experiment: samples, gates,
annotations, statistics, compensation matrices, all your graphs, and
anything else you need for your data analysis. Once you set up the
workspace, data analysis is very fast. A workspace can actually be used as
a 'template' for batch analysis of any subsequent experiment (this makes
life so much easier!!).
The data with any statistic you can possibly wish for can be
transferred to Excel with a few clicks of the mouse: basically you choose
the statistics you're interested in, and FlowJo will create a spreadsheet
for you with all the calculated statistics from the samples you have
selected. The spreadsheet can then be easily exported to Excel or to
another application.
If you are staining with multiple markers, FlowJo will perform
compensation correctly, no matter what the number of your fluorochromes is
(it is virtually impossible to set compensation correctly with the
'eye-meter' when your samples have been stained for multiple markers).
The one and only criticism is that you need a Mac to run it. But
after starting to use it we decided it was actually worth to buy a new Mac,
which is now devoted only to data analysis with FlowJo (so we don't have to
fight over the Mac connected to the FACS...).
It doesn't take too long to learn - of course, you have to make a
time investment, but it will really pay off. The Help files are helpful
indeed - it took us a couple of days, and that was it. You can try it for a
couple of months by downloading the software and getting a free serial
number. Go for it.
I will be glad to show you some of the data we have produced
(FlowJo even creates movies!!) if you are interested.
Good luck!
Giovanna
(a FlowJo enthusiast)
>> Hi:
>>
>> I wanted some feedback on the FLOW Jo application. I
>>currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
>>may expedite my analysis. However, I do not use the same markers all the
>>time, and in one experiment I may be running more than one stain with
>>multiple different antibodies. Basically, very seldom do I run the same set
>>of markers. How does FLOW Jo work for such conditions?? How about plot
>>analysis and excel: apparently Excell FLOW Jo puts the data directly into
>>EXcell?? What has been your experience with FLOW Jo?? Any information would
>>be useful.
>>
>> denise
------------------------------------------------
Giovanna Borsellino, M.D., Ph.D.
Neuroimmunology
IRCCS Santa Lucia
Via Ardeatina 354
00179 Roma
Italy
Tel. ++39.06.51501521 (office)
++39.06.51501552 (lab)
Fax ++39.06.51501553
e-mail: g.borsellino@hsantalucia.it
gborsel@tin.it
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: janet dow (jldow@unity.ncsu.edu)
Date: Wed Jun 20 2001 - 11:27:00 EST
I have recently started using flowjo and i love it. You can go to their
web site, www.flowjo.com and you can get a free 60 day trial on the
software. I totally recomment trying it.
Janet Dow
At 10:37 AM -0400 6/19/01, Manfra, Denise wrote:
> Hi:
>
> I wanted some feedback on the FLOW Jo application. I
>currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
>may expedite my analysis. However, I do not use the same markers all the
>time, and in one experiment I may be running more than one stain with
>multiple different antibodies. Basically, very seldom do I run the same set
>of markers. How does FLOW Jo work for such conditions?? How about plot
>analysis and excel: apparently Excell FLOW Jo puts the data directly into
>EXcell?? What has been your experience with FLOW Jo?? Any information would
>be useful.
>
> denise
>
>***************************************************************
> This message and any attachments is solely for the intended recipient. If
>you are not the intended recipient, disclosure, copying, use, or
>distribution of the information included in this message is prohibited --
>please immediately and permanently delete this message.
Janet Dow
Research Technician and Manager
Flow Cytometry Facility
North Carolina State College of Veterinary Medicine
Room C-314
Raleigh, NC 27606
(919)513-6364
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Maciej Simm (simmmmer@yahoo.com)
Date: Wed Jun 20 2001 - 17:01:17 EST
Denise,
Analysis in FlowJo works faster than other programs with correct
setup and the right template.
Regardless of how many markers you use, FlowJo will automatically
detect your panel settings and you can isolate/sort FACS files based
on their staining protocol (or any keyword) for a separate analysis.
Once you analyse some files you can generate statistical tables which
can be copied to clipboard and/or opened in excel.
For example, if I have an analyzed set of 1,000 samples for oxidative
burst test and want to generate an excel spreadsheet of
name/date/geometric mean/%positive and CV, I would specify those
parameters in my table editor, and with 2 click of a mouse I'll be in
excel ready to print the results.
FlowJo is also a great database tool for FACS files - one application
of that is to find normal ranges of rarely used markers. add a years
worth of FCS filies (provided enough RAM), create a group based on
your marker, apply gates to all of them with one click, and .. voila.
Plots can also be copied/pasted (as well as exported) into
Word/Excel/PowerPoint/Canvas etc.
Feel free to contact me if you have any other questions,
Maciej Simm
Tree Star, Inc.
__________________________________________________
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
RE: FlowJo
From: CJett (CJett@Compucyte.com)
Date: Wed Jun 20 2001 - 15:25:00 EST
Most hardely agree with Adrian Smith...Flow Jo is a time saver for SO many
parts of data analysis. It does take some time to get used to if you've
been working in the Cellquest reality for long..but well worth it! Just not
having to enter data by hand is reason enuff! But I do suggest keeping CQ
around...graphically it is a lil simpler to cut and paste from
Cj Jett
Scientist: Biomedical Development
Compucyte Corp.
(617)-577-3811
12 Emily St.
Cambridge Ma 02139-4507
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Phil Marder (mr_redram@hotmail.com)
Date: Thu Jun 21 2001 - 14:39:16 EST
When is the PC version of FloJo coming?
Some of us are waiting for this to give it a try.
-Phil Marder
----Original Message Follows----
From: Maciej Simm <simmmmer@yahoo.com>
To: cyto-inbox
Subject: Re: FlowJo
Date: Wed, 20 Jun 2001 15:01:17 -0700 (PDT)
Denise,
Analysis in FlowJo works faster than other programs with correct
setup and the right template.
Regardless of how many markers you use, FlowJo will automatically
detect your panel settings and you can isolate/sort FACS files based
on their staining protocol (or any keyword) for a separate analysis.
Once you analyse some files you can generate statistical tables which
can be copied to clipboard and/or opened in excel.
For example, if I have an analyzed set of 1,000 samples for oxidative
burst test and want to generate an excel spreadsheet of
name/date/geometric mean/%positive and CV, I would specify those
parameters in my table editor, and with 2 click of a mouse I'll be in
excel ready to print the results.
FlowJo is also a great database tool for FACS files - one application
of that is to find normal ranges of rarely used markers. add a years
worth of FCS filies (provided enough RAM), create a group based on
your marker, apply gates to all of them with one click, and .. voila.
Plots can also be copied/pasted (as well as exported) into
Word/Excel/PowerPoint/Canvas etc.
Feel free to contact me if you have any other questions,
Maciej Simm
Tree Star, Inc.
__________________________________________________
Do You Yahoo!?
Get personalized email addresses from Yahoo! Mail
http://personal.mail.yahoo.com/
_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Thu Jun 21 2001 - 16:44:49 EST
At 10:26 AM +0100 21/6/2001, Giovanna Borsellino wrote:
>
> The one and only criticism is that you need a Mac to run it.
>
Some people would say that is a definite advantage :)
At 4:25 PM -0400 20/6/2001, CJett wrote:
> But I do suggest keeping CQ
>around...graphically it is a lil simpler to cut and paste from
But plots looks SO much nicer when they come from FlowJo...
Adrian Smith
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
Re: FlowJo
From: Carmen Raventos-Suarez (carmen.raventossuarez@bms.com)
Date: Thu Jun 21 2001 - 16:49:26 EST
Denise and everybody else interested in Flow Jo:
I have been using Flow Jo for a year and my requirements are very much like
yours. I also do a lot of DNA analysis, apoptosis and proliferation assays.
We just use the Fl-1, Fl-2, etc. as defaults. That way we can run different
set of samples using several different markers with the same dye at a single
run. Later in the analysis we add to the plots the specific name of the
markers. It is fast and efficient, I definitely love it, my analysis time has
been cut down in an exponential way. You create a template and can drop buckets
of samples in it to be analyzed in no time. This includes time for you to view
every single plot in a movie sequence to be sure that all your gates are
correct. Still if you have cells from different origins and need to correct
gates, this can be done for the individual samples that require correction
leaving the rest untouchable. And the results on excel are at your finger tips
customized by your needs in a couple of clicks. I will never forget all the
macros I needed to built to create individual excel tables from cell quest.
I fewer words, as I told BD who where the ones who introduce me to Flow Jo:
"Flow Jo is like to live in the future and there is no way you want to return
to the past".
Carmen
"Manfra, Denise" wrote:
> Hi:
>
> I wanted some feedback on the FLOW Jo application. I
> currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
> may expedite my analysis. However, I do not use the same markers all the
> time, and in one experiment I may be running more than one stain with
> multiple different antibodies. Basically, very seldom do I run the same set
> of markers. How does FLOW Jo work for such conditions?? How about plot
> analysis and excel: apparently Excell FLOW Jo puts the data directly into
> EXcell?? What has been your experience with FLOW Jo?? Any information would
> be useful.
>
> denise
>
> ***************************************************************
> This message and any attachments is solely for the intended recipient. If
> you are not the intended recipient, disclosure, copying, use, or
> distribution of the information included in this message is prohibited --
> please immediately and permanently delete this message.
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST
RE: FlowJo
From: Gerstein, Rachel (Rachel.Gerstein@umassmed.edu)
Date: Fri Jun 22 2001 - 09:39:24 EST
I think FlowJo will still expedite your analysis, because its quick to call
up new 2-D plots and impose new gates. And the quick dump into Excell, or
using FlowJo's own table generator will also save time. I use FlowJo
exclusively and highly recommend it. Its worth mentioning that its easy to
learn and I rountinely teach undergraduates to do their own analyses.
Good luck,
Rachel
=======================================================
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)
> ----------
> From: Manfra, Denise
> Sent: Tuesday, June 19, 2001 10:37 AM
> To: Cytometry Mailing List
> Subject: FlowJo
>
>
>
> Hi:
>
> I wanted some feedback on the FLOW Jo application. I
> currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
> may expedite my analysis. However, I do not use the same markers all the
> time, and in one experiment I may be running more than one stain with
> multiple different antibodies. Basically, very seldom do I run the same
> set
> of markers. How does FLOW Jo work for such conditions?? How about plot
> analysis and excel: apparently Excell FLOW Jo puts the data directly into
> EXcell?? What has been your experience with FLOW Jo?? Any information
> would
> be useful.
>
> denise
>
> ***************************************************************
> This message and any attachments is solely for the intended recipient. If
> you are not the intended recipient, disclosure, copying, use, or
> distribution of the information included in this message is prohibited --
> please immediately and permanently delete this message.
>
>
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:40:22 EST